Inter-relação entre lesão cervical não cariosa e comprometimento periodontal: diagnóstico, tratamento e previsibilidade - estudo clínico prospectivo e laboratorial

The coexistence of gingival recession (GR) with root coverage indication and non-carious cervical lesions (LCNC) generates the need for a protocol that respects and promotes health of dental and periodontal tissues and allows treatment predictability. The main objectives of this theses were: (1) verify, through clinical evaluations, the connective tissue graft for root coverage on direct and indirect restorations made of ceramic resin; (2) analyze the influence of the battery level of the LED curing unit in the composite resin characteristics; (3) assess the influence of restorative materials, composite resin and ceramics, on the viability of gingival fibroblasts from primary culture. Nine patients with good oral hygiene and occlusal stability diagnosed with LCNCs the anterior teeth including premolars associated with gingival recession (class I and II of Miller) and only gingival recession were selected. After initial clinical examination, occlusal adjustment was performed and the patients had their teeth randomized allocated on direct composite resin restoration of LCNC, polishing and GR treatment with connective tissue graft and advanced coronally flap CR group (n = 15); and indirect ceramic restoration of the LCNC's and GR treatment (CTG+CAF) Group C (n = 15). The GR presented teeth with no clinically formed LCNCs cavity were treated using (CTG+CAF) being the control group (n = 15). Sorption and solubility tests, analysis of the degree of conversion and diametral tensile strength were performed in composite resin samples (n = 10) photoactivated by 100, 50 and 10% battery charge LED unit. The viability of fibroblasts on composite resin, ceramics and dentin disks (n = 3) was examined. Clinical follow-up was performed for three months. The data obtained at different stages were tabulated and subjected to analysis for detection of normal distribution and homogeneity. The results showed that: the LED unit with 10% battery affects the characteristics of the composite resin; restorative materials present biocompatibility with gingival fibroblasts; and the association of surgical and restorative treatment of teeth affected by NCCL and GR presents successful results at 3-month follow-up.

Utilização de peptídeos sintéticos derivados de moléculas imunodominantes de Toxoplasma gondii para diagnóstico sorológico da toxoplasmose em ovinos e galinhas caipiras

Chapter I - The obligate intracellular parasite Toxoplasma gondii is the causative agent of toxoplasmosis, a disease that affects humans and generates economic losses in farm animals. When prevention fails disease refers to the diagnosis and subsequent treatment if the individual is diagnosed as positive. Therefore, the development of new accurate diagnostic tools for detecting T. gondii infection is a need in particular to determine the environmental source of infection which can result in more appropriate public health policies against different routes of infection and prevent potential damage that toxoplasmosis can cause when animals are infected. Chapter II - The domestic chicken (Gallus gallus domesticus) are considered epidemiological sentinels, still representing a major source of recombinant strains when predated by cats, it is common to find them with multiple infections. We evaluate the diagnostic potential of six synthetic peptides (SAG2Y, MIC1, M2AP, GRA10, ROP2 and ROP7) predicted in silico from tachyzoites immunodominant markers of T. gondii in samples from naturally infected chickens, comparing synthetic peptides with antigen total soluble (STAg). In general, our results demonstrated that reactivity rates and positivity for these peptides are similar to the STAg, and the ROP7 peptide and the combination of peptides MIC1+ROP2 have significant sensitivity, confirming them as potential diagnostic tools for the diagnosis of toxoplasmosis in chickens. Chapter III - Sheep (Ovis aries) are commonly infected with Toxoplasma gondii due to his eating habits. Infection in pregnant sheep can have serious consequences such as embryonic death, fetal resorption, mummification, and neonatal death. One concern regarding the infection in these animals is that the meat can be a source of contamination to humans and other carnivores. Therefore perform accurate diagnosis in these animals is of fundamental importance. In the present study we evaluated the potential of new synthetic peptides as a diagnostic tool. Synthetic peptides (SAG2Y, SRS52A, MIC14, GRA4, GRA10 and GRA15) were predicted in silico from immunodominant proteins of T. gondii. We determine the levels of IgG antibodies using sera obtained from two farms in the city of Uberlândia. Analyzing the results together, the peptide combination GRA10+GRA15 (Accuracy = 0,82) showed better characteristics compared with the other mixtures. This preparation could be better analyzed with an antigenic mixture potential use in the diagnosis of toxoplasmosis in sheep and other species.

Diagnóstico ambiental da área de influência do complexo sucroalcooleiro Usina Vale do São Simão Ltda

One of the most widespread renewable energy sources in Brazil is ethanol, from sugarcane, therefore, the sugar and alcohol sector is expanding, with positive impacts for the economy of the country. Sugar cane was introduced in Brazil as a crop during its colonization, for the production of sugar, and put the country in the global scenario. The expansion of this crop occurred in the seventies, to reduce the reliance in fossil energy sources and to stimulate the development of the agricultural activity. Thus, the federal government has promoted the sugar cane crop and the production of ethanol as a fuel. However, it is important to minimize possible impacts that the crop may cause to the environment. Sugar cane has expanded in the frontiers of the mesoregion of Triângulo Mineiro and Alto Paranaíba-MG, and, in this perspective, the agroindustrial complex known as Companhia Energética Vale do São Simão Ltda., with the Mill located in the county of Santa Vitória, Minas Gerais, was adopted to evaluate the environmental impacts caused by the sugarcane in the area of influence of the mill. The mill has a polygonal area corresponding to 53,525.20 hectares, and for its establishment a Study and Report of Environmental Impacts (EIA/RIMA) was presented, as required as an environment protection instrument by the Environment National Policy (Law nº 6.938/81), and detailed by the Resolution CONAMA nº 01/1986. These studies pointed that native vegetation fragments in the Area of Influence of the Mill, before its implantation, corresponded to approximately 20.7% of the area. Therefore, this study evaluated the impacts of the installation of Usina Vale do São Simão, between 2007 and 2012, determining its reflex on the environmental regularization of the farms, and the vegetation fragments existing in the area, in the recovery and recomposition of areas defined as Legal Reserve and Permanent Preservation. Previous studies of the area were analyzed, soil use and occupation was mapped for the years 2007 and 2012, and the areas of permanent preservation and native vegetation fragments were marked. In general, there was a decline in native vegetation coverage in the period, although it cannot be stated that such reduction was a direct effect of the milling activity. Therefore, the legal requirement of preserving such areas was not capable of bringing the positive effects of protection and recovery as demanded by the Law, highlighting that the current legislation was not enough to protect such areas.